Отрывок: For example, fluorescence nanoscopy (STORM) recently revealed that actin, spectrin and accompanying proteins in axons form a membrane-associated periodic skeleton (MPS) arranged as periodic actin rings separated by 180 nm – 190 nm, [49, 50]. Nevertheless, as de- scribed in [50] this structure is not always present in all axons. This type of structures will cross the frame bound- ary and would present artifact...
Название : | Solving the boundary artifact for the enhanced deconvolution algorithm SUPPOSe applied to fluorescence microscopy |
Авторы/Редакторы : | Toscani, M. Martínez, S. |
Ключевые слова : | deconvolution fluorescence microscopy boundary artifact |
Дата публикации : | Июн-2021 |
Издательство : | Самарский национальный исследовательский университет имени акад. С.П. Королева |
Библиографическое описание : | Toscani M, Martínez S. Solving the boundary artifact for the enhanced deconvolution algorithm SUPPOSe applied to fluorescence microscopy. Computer Optics 2021; 45(3): 418-426. DOI: 10.18287/2412-6179-CO-825. |
Серия/номер : | 45;3 |
Аннотация : | The SUPPOSe enhanced deconvolution algorithm relies in assuming that the image source can be described by an incoherent superposition of virtual point sources of equal intensity and finding the number and position of such virtual sources. In this work we describe the recent advances in the implementation of the method to gain resolution and remove artifacts due to the presence of fluorescent molecules close enough to the image frame boundary. The method was modified removing the invariant used before given by the product of the flux of the virtual sources times the number of virtual sources, and replacing it by a new invariant given by the total flux within the frame, thus allowing the location of virtual sources outside the frame but contributing to the signal inside the frame. |
URI (Унифицированный идентификатор ресурса) : | https://dx.doi.org/10.18287/2412-6179-CO-825 http://repo.ssau.ru/handle/Zhurnal-Komputernaya-optika/Solving-the-boundary-artifact-for-the-enhanced-deconvolution-algorithm-SUPPOSe-applied-to-fluorescence-microscopy-89754 |
Другие идентификаторы : | Dspace\SGAU\20210620\89754 |
Располагается в коллекциях: | Журнал "Компьютерная оптика" |
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450314.pdf | Основная статья | 1.29 MB | Adobe PDF | Просмотреть/Открыть |
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